Desired GC content (%):
20 |
30 |
40 |
50 |
60 |
70 |
80 |
90 |
100
Number of nucleotides (NT):
(max. 1000) Sequence (5' to 3'):
TAATGTGCGATTTTTTTGAAT
[dT to rU]
GC content: 23.8 %, T
m
: 42.6°C
(Assumption: 50 nM oligo, 50 mM Na
+
at pH 7.0 - based on this
link
)
Click ↑↑↑ to get scrambled DNA sequences
by
Minseok Kwak
Click ↑↑↑ to get scrambled DNA sequences