oligorand v2.0 (JavaScript edition — all generation is local in your browser)
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• Desired GC content (%):
• Base type:
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(max. 1000)
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Tm assumption: 50 nM oligo, 50 mM Na+ at pH 7.0
(based on this link).
GC% is approximate per sequence (random sampling from a GC-weighted pool).
Tm = DNA approximation (RNA-specific NN model not used. You should double-check the estimated Tm).
