Desired GC content (%):
30 |
40 |
50 |
60 |
70 |
80 |
90 |
100
Number of nucleotides (NT):
(max. 1000)
GC content: 47.6 %, T
m
: 52.4°C
(Assumption: 50 nM oligo, 50 mM Na
+
at pH 7.0 - based on this
link
)
Sequence (5' to 3'):
TTACACTAGCAAGCTTAGCGC
Click ↑↑↑ to get scrambled DNA sequences
by
Minseok Kwak
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Click ↑↑↑ to get scrambled DNA sequences