v1.51
Desired GC content (%):
20 |
30 |
40 |
50 |
60 |
70 |
80 |
90 |
100
Number of nucleotides (NT):
(max. 1000) Sequence (5' to 3'):
TAAATCTTTATTTATACTTAA
✅ save
GC content: 9.5 %, T
m
: 36.8°C
(Assumption: 50 nM oligo, 50 mM Na
+
at pH 7.0 - based on this
link
)
[dT to rU]
Saved sequence:
by
Minseok Kwak
v1.51