History: 20260614

oligorand v2 — New Features Summary

Conversation: mkwak

Date: 2026-06-14

Target file: oligorand2_upload.html (mkwak.org, JavaScript edition)

A summary of features added/improved in the in-browser, locally-generated random oligo generator.

1. Saved sequences

Cookie-based storage: generated sequences are kept in a browser cookie (oligo_saved), up to 10 entries for 90 days.
Each row provides copy / delete (✕) buttons.
Duplicate sequences are not saved; an alert is shown when the 10-entry limit is exceeded.
Saved entries display the sequence, NT (length), GC %, and Tm.
Delete all sequences in cookie button: shown only when saved entries exist; clicking it opens a confirm() dialog ("Delete all N saved sequences…") and clears the cookie / refreshes the list only if confirmed.

Generated sequences table (.gentbl): grey header (#eee) only, white body.
Saved sequences table (.savedtbl): white grid lines + per-column pastel backgrounds (blue/purple/green/orange); the action column (6th) is grey in both header and body.
parambox (parameter input box): light grey (#eee) background + grey border.

GC content radio selection (20–100%), random sampling from a GC-weighted pool.
Base type switching: DNA (dT) ↔ RNA (rU) selection, with per-row dT→rU / rU→dT conversion buttons (with confirm() prompt).
NT length (up to 1000) and number of sequences (choose from 1·2·4·8·16).
Run highlighting: runs of 4+ identical bases (AAAA/CCCC/GGGG/TTTT) are color-marked.
Tm calculation: Wallace rule below 14 nt, salt-adjusted formula above; a "Tm = DNA approximation" warning is shown when RNA is selected.
GC % calculation and 5′→3′ direction labeling.

Uses the Web Crypto CSPRNG (crypto.getRandomValues).
Rejection sampling removes the modulo bias of rand()%n (guarantees a uniform distribution).

Per-sequence copy and Copy all sequences (copies to clipboard as tab-separated Sequence/GC%/Tm text).
Non-secure (http) compatibility: navigator.clipboard is exposed only on HTTPS·localhost, so the Clipboard API is used in a secure context and automatically falls back to a hidden textarea + execCommand("copy") when it fails or on http (copyText()/legacyCopy()). → Copy works even when served over http in Safari·Firefox.

On page load, the top image transitions from blur→sharp over 1 second (@keyframes sharpenIn, from blur(14px) brightness(3) to blur(0) brightness(1)).

An "Update history »" link next to the title (target="_blank", rel="noopener") opens the history in a new window.
history.php: a viewer that scans *.md in the same folder in newest-first order, parses them, and renders them as cards. Compatible with both PHP 5 and PHP 8 (no removed syntax used); ?f=filename.md selects a specific file, defaulting to the newest.

Image components from Microsoft Office resource. by Minseok Kwak